Difference between revisions of "Part:BBa C0020:Design"

Revision as of 01:22, 5 June 2006

CheY "tumbling" chemotaxis coding sequence

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Changes from the NCBI sequence: A BioBricks restriction site for PstI was at position 260. As a result, the codon starting at position 262 was changed from "gca" to "gcg".

The terminating codon at the end, "tga", was changed to "taataa" as per BioBrick regulations as told to us by Randy Rettberg.

Source

NCBI - Escherichia coli K12 MG1655 (NC_000913)

References

<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=946393">NCBI sequence data</a>

@@ Line 5: / Line 5: @@
 <partinfo>BBa_C0020 SequenceAndFeatures</partinfo>
-===Design Notes===
+===Design Notes===
 Changes from the NCBI sequence:
 A BioBricks restriction site for PstI was at position 260. As a result, the codon starting at position 262 was changed from "gca" to "gcg".
 The terminating codon at the end, "tga", was changed to "taataa" as per BioBrick regulations as told to us by Randy Rettberg.
 ===Source===
---What is the source of this part?--
+NCBI - Escherichia coli K12 MG1655 (NC_000913)
 ===References===
 * <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=946393">NCBI sequence data</a>
-===Files===