Difference between revisions of "Part:BBa K422014:Design"

Latest revision as of 10:28, 27 October 2010

mCyPet (AarI A-part)

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 733
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 733
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 733
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 733
Illegal NgoMIV site found at 697
1000
COMPATIBLE WITH RFC[1000]

Design Notes

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. mCyPet-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].

Source

Codon optimized.

References

http://2010.igem.org/Team:ETHZ_Basel

Ohashi, Galiacy, Briscoe and Erickson: An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins. Protein Science. 2007; 16.

Nguyen and Daugherty: Evolutionary optimization of fluorescent proteins for intracellular FRET. Nature Biotechnology. 2005; 23.

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K422014 short</partinfo>
@@ Line 7: / Line 6: @@
 ===Design Notes===
-xxx
+BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. mCyPet-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].
+===Source===
+Codon optimized.
+===References===
+http://2010.igem.org/Team:ETHZ_Basel
-===Source===
+Ohashi, Galiacy, Briscoe and Erickson: An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins. Protein Science. 2007; 16.
-xxx
+Nguyen and Daugherty: Evolutionary optimization of fluorescent proteins for intracellular FRET. Nature Biotechnology. 2005; 23.
-===References===