Difference between revisions of "Part:BBa K337003"
 
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<partinfo>BBa_K337003 short</partinfo>
 
<partinfo>BBa_K337003 short</partinfo>
  
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hsa-miR-122 binding site pattern, consisting of 4 binding sites (BS). The BS are seperated by sequences of 30 base pairs, which are inert in terms of miRNA binding. Additionally, half of the separation sequence is either in front or the end of the whole pattern, to be unconnected to the standard restriction sites.
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hsa-miR-122 binding site pattern, consisting of 4 binding sites (BS). The BS are seperated by sequences of 30 base pairs, which are inert in terms of miRNA binding. Additionally, half of the separation sequence is either in front or the end of the whole pattern, to be unconnected to the standard restriction sites (BB-2, RFC12).
  
  

Latest revision as of 09:34, 27 October 2010

hsa-miR-122 Binding site pattern (4BS)

hsa-miR-122 binding site pattern, consisting of 4 binding sites (BS). The BS are seperated by sequences of 30 base pairs, which are inert in terms of miRNA binding. Additionally, half of the separation sequence is either in front or the end of the whole pattern, to be unconnected to the standard restriction sites (BB-2, RFC12).


Mutations showed up for BS 1 and 2, where BS 3 and 4 are perfectly matching:

1st: CAAACACCATTGTCACACTC-A
perfect: CAAACACCATTGTCACACTCCA
2nd: CAAACACCATTGTCA-ACTCCA
perfect: CAAACACCATTGTCACACTCCA


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]