Difference between revisions of "Part:BBa K337041"
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<partinfo>BBa_K337041 short</partinfo> | <partinfo>BBa_K337041 short</partinfo> | ||
| − | CMV promoter driving a Tet Repressor followed by BGH terminator. The construct is flanked by rc BGH terminators to introduce BamHI sites. XhoI/XmaI sites were introduced into the 3'UTR of the TetR for enabling easy introduction of synthetic microRNA binding sites. | + | CMV promoter driving a Tet Repressor followed by BGH terminator. The construct is flanked by rc BGH terminators to introduce BamHI sites. XhoI/XmaI sites were introduced into the 3'UTR of the TetR for enabling easy introduction of synthetic microRNA binding sites (BB-2, RFC12). |
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Latest revision as of 09:21, 27 October 2010
BGH(rc)_CMV/TetR/BGH(fw)/BGH(rc)
CMV promoter driving a Tet Repressor followed by BGH terminator. The construct is flanked by rc BGH terminators to introduce BamHI sites. XhoI/XmaI sites were introduced into the 3'UTR of the TetR for enabling easy introduction of synthetic microRNA binding sites (BB-2, RFC12).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]