Difference between revisions of "Part:BBa K337039"
 
Line 2: Line 2:
 
<partinfo>BBa_K337039 short</partinfo>
 
<partinfo>BBa_K337039 short</partinfo>
  
−
Mutated TetR where XhoI/XbaI sites were introduced right after the stop codon enabling easy introduction of synthetic microRNA binding sites into the 3'UTR of the TetR.
+
Mutated TetR where XhoI/XbaI sites were introduced right after the stop codon enabling easy introduction of synthetic microRNA binding sites into the 3'UTR of the TetR (BB-2, RFC12).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 09:20, 27 October 2010

TetR_mut(XhoI/XbaI)

Mutated TetR where XhoI/XbaI sites were introduced right after the stop codon enabling easy introduction of synthetic microRNA binding sites into the 3'UTR of the TetR (BB-2, RFC12).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 652
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]