Difference between revisions of "Part:BBa K395304"

Latest revision as of 09:18, 27 October 2010

PompC(C) with GFP reporter (K395301+E0240)

This part is contructed for measuring the transcriptional activity of OmpC(C) promoter through the GFP expression. The promoter of this part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about PompC series]

Figure 1. Induction of new OmpC series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA Tokyo Tech iGEM2010

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 24
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 734

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	This part is contructed for measuring the transcriptional activity of [https://parts.igem.org/Part:BBa_K395301 ''OmpC(C)''] promoter through the GFP expression. The promoter of this part is positively regulated by phosphorylated ''OmpR'', a response regulator in EnvZ-''OmpR'' two-component system [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about P''ompC'' series]		This part is contructed for measuring the transcriptional activity of [https://parts.igem.org/Part:BBa_K395301 ''OmpC(C)''] promoter through the GFP expression. The promoter of this part is positively regulated by phosphorylated ''OmpR'', a response regulator in EnvZ-''OmpR'' two-component system [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about P''ompC'' series]
		+	[[Image:Tokyotech_ompc_graph.jpg\|thumb\|center\|400px\|Figure 1. Induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA Tokyo Tech iGEM2010]]