Difference between revisions of "Part:BBa K313018"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K313018 short</partinfo> | <partinfo>BBa_K313018 short</partinfo> | ||
| − | This is a device that aims to measure the leakiness of terminator BBa_B1006. | + | This is a device that aims to measure the leakiness of a terminator BBa_B1006. |
| + | If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part. | ||
| + | With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, we can detect the quantity of Cre and can assess the leakiness of the terminator. | ||
| + | The difference between this part and BBa K313017 is the number of the terminator. | ||
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Revision as of 07:40, 27 October 2010
terminator leakiness assay device
This is a device that aims to measure the leakiness of a terminator BBa_B1006. If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part. With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, we can detect the quantity of Cre and can assess the leakiness of the terminator. The difference between this part and BBa K313017 is the number of the terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 525
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 211
- 1000COMPATIBLE WITH RFC[1000]