Difference between revisions of "Part:BBa K395304"
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This part is contructed for measuring the transcriptional activity of [https://parts.igem.org/Part:BBa_K395301 ''OmpC(C)''] promoter through the GFP expression. The promoter of this part is positively regulated by phosphorylated ''OmpR'', a response regulator in  EnvZ-''OmpR'' two-component system [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about P''ompC'' series]
 
This part is contructed for measuring the transcriptional activity of [https://parts.igem.org/Part:BBa_K395301 ''OmpC(C)''] promoter through the GFP expression. The promoter of this part is positively regulated by phosphorylated ''OmpR'', a response regulator in  EnvZ-''OmpR'' two-component system [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about P''ompC'' series]
 +
[[Image:Tokyotech_ompc_graph.jpg|thumb|center|400px|Figure 1. Induction of new'' OmpC'' series in high osmolarity medium  This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA Tokyo Tech iGEM2010]]
  
  

Revision as of 06:09, 27 October 2010

PompC(C) with GFP reporter (K395301+E0240)

This part is contructed for measuring the transcriptional activity of OmpC(C) promoter through the GFP expression. The promoter of this part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about PompC series]

Figure 1. Induction of new OmpC series in high osmolarity medium This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA Tokyo Tech iGEM2010


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 24
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734