Difference between revisions of "Part:BBa K395705"
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[[Image:tokyotech_Fig. 2-1-11-2 astaxanthin pellet and plate.jpg|right|460px|Fig. 2-1-11-2 astaxanthin pellet and plate]] | [[Image:tokyotech_Fig. 2-1-11-2 astaxanthin pellet and plate.jpg|right|460px|Fig. 2-1-11-2 astaxanthin pellet and plate]] | ||
| − | [[Image:tokyotech_Fig. 2-1-12-2 astaxanthin extract.jpg|right| | + | [[Image:tokyotech_Fig. 2-1-12-2 astaxanthin extract.jpg|right|500px|Fig. 2-1-12-2 astaxanthin extract]] |
[[Image:tokyotech_table. 2.1.3.jpg|left|450px|figure2]] | [[Image:tokyotech_table. 2.1.3.jpg|left|450px|figure2]] | ||
[[Image:tokyotech_Fig. 2-1-1-3.TLC.jpg|left|450px|Fig. 2-1-1-3.TLC.jpg]] | [[Image:tokyotech_Fig. 2-1-1-3.TLC.jpg|left|450px|Fig. 2-1-1-3.TLC.jpg]] | ||
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Revision as of 05:36, 27 October 2010
crtW (beta-carotene ketolase) with rbs
CrtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.
Part of the carotenoid biosynthesis pathway. CrtW converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin.
These parts contain crtW (β-carotene ketolase) under inducible promoter control. This enzyme is responsible for the conversion of zeaxanthin into astaxanthin. Our team designed two BioBricks related to this conversion. Characterization of BBa_K395706 has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 632
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 715
Illegal SapI site found at 542
Illegal SapI site found at 554