Difference between revisions of "Part:BBa K349010:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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This part has built-in BbsI cutsites surrounding the p1003 kanamycin resistance cassette. Digestion at these sites produces an A overhang at the 5' end and a B overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method.
+
This part has built-in BbsI cutsites surrounding the p1003 kanamycin resistance cassette. Digestion at these sites produces a B overhang at the 5' end and an A overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method.
  
 
===Source===
 
===Source===

Latest revision as of 03:07, 27 October 2010

KanR-BA-BbsI (for construction of BA BioBytes 2.0)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has built-in BbsI cutsites surrounding the p1003 kanamycin resistance cassette. Digestion at these sites produces a B overhang at the 5' end and an A overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method.

Source

pSB1C3

References