Difference between revisions of "Part:BBa K325249"

 
Line 1: Line 1:
−
 
+
{{:Templates/Cambridge-fireflycolour|colour=scarlet|substitution=His433Tyr|id=249}}
−
__NOTOC__
+
−
<partinfo>BBa_K325249 short</partinfo>
+
−
 
+
−
a
+
−
 
+
−
<!-- Add more about the biology of this part here
+
−
===Usage and Biology===
+
−
 
+
−
<!-- -->
+
−
<span class='h3bb'>Sequence and Features</span>
+
−
<partinfo>BBa_K325249 SequenceAndFeatures</partinfo>
+
−
 
+
−
 
+
−
<!-- Uncomment this to enable Functional Parameter display
+
−
===Functional Parameters===
+
−
<partinfo>BBa_K325249 parameters</partinfo>
+
−
<!-- -->
+

Revision as of 02:58, 27 October 2010

Input: L-Arabinose
Output: Light (scarlet)

pBad/araC
I0500		mutated version of
Luciferase/LRE
K325210

This part should show similarity to BBa_K325219     Add Data       


Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a His433Tyr mutation was used to create a scarlet bioluminescence.


Colour of emission

Figure 1 - Comparison of part BBa_K325249 against other constructs created by the E.glowli team. Note that this picture should be used as a relative comparison rather than absolute colour. All images change markedly depending on the white balance used.


Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3