Difference between revisions of "Templates/Cambridge-fireflycolour"
(→Pictures) |
|||
| Line 19: | Line 19: | ||
<div class="noprint" style="padding: 10px; color: #ffffff; background-color: #C0C0C0; width: 660px; align: center"> | <div class="noprint" style="padding: 10px; color: #ffffff; background-color: #C0C0C0; width: 660px; align: center"> | ||
<center> | <center> | ||
| − | <span style="color:black">This part should show similarity to</span> [https://parts.igem.org/Part:BBa_K325219 BBa_K325219] [[Part: | + | <span style="color:black">This part should show similarity to</span> [https://parts.igem.org/Part:BBa_K325219 BBa_K325219] [[Part:BBa_K325{{{id}}}:Experience |Add Data]] |
</center> | </center> | ||
</div><br> | </div><br> | ||
Revision as of 02:56, 27 October 2010
|
|
|
Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase]. Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.
Pictures
Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with BBa K325909 (blue light bulb) and BBa 325219 (red light bulb)
1Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]