Difference between revisions of "Part:BBa K337021"

 
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<partinfo>BBa_K337021 short</partinfo>
 
<partinfo>BBa_K337021 short</partinfo>
  
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SV40 promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, CMV promoter near SV40 drives in the opposite direction (fw).
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SV40 promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator (reverse complementary). The cDNA of the users' choice can be introduced behind the CMV promoter. (BB-2, RFC12)  
  
 
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Revision as of 02:33, 27 October 2010

BGH(rc)/shRNA10(rc)/sv40(rc)/CMV(fw)

SV40 promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator (reverse complementary). The cDNA of the users' choice can be introduced behind the CMV promoter. (BB-2, RFC12)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]