Difference between revisions of "Part:BBa R0065"

Revision as of 02:17, 27 October 2010

Promoter (lambda cI and luxR regulated -- hybrid)

cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR.

LuxI	cI	activity of promoter
+	+	zero
+	-	maximum
-	+	zero
-	-	leaky (no quantitative information)

Usage and Biology

Pretty good 'off' in the absence of LuxR/HSL. [jb, 5/24/04]

very leaky (no LuxR/HSL) at high (100-300) copy number. can only be slightly induced by LuxR+3OC6HSL. (jt, ut-austin; 08/11/05)
Part has since been improved by the [http://2010.igem.org/Team:MIT 2010 MIT iGEM] team in the form of the part Part:BBa_K415031 where the second -10 promoter region has been removed, and the part is thus less leaky.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_R0065 short</partinfo>
@@ Line 15: / Line 14: @@
 *very leaky (no LuxR/HSL) at high (100-300) copy number.  can only be slightly induced by LuxR+3OC6HSL.  (jt, ut-austin; 08/11/05)
+* Part has since been improved by the [http://2010.igem.org/Team:MIT 2010 MIT iGEM] team in the form of the part [[Part:BBa_K415031]] where the second -10 promoter region has been removed, and the part is thus less leaky.
 <span class='h3bb'>Sequence and Features</span>