Difference between revisions of "Part:BBa K395704"
| Line 5: | Line 5: | ||
It is able to control the expression of crtZ by arabinose. | It is able to control the expression of crtZ by arabinose. | ||
| + | |||
| + | [[Image:tokyotech_Fig. 1.1.8.1 zeaxanthin pellet and plate.jpg|right|460px|Fig. 1.1.8.1 zeaxanthin pellet and plate]] | ||
| + | [[Image:tokyotech_table. 1.1.1.2.jpg|left|450px|figure1]] | ||
| + | |||
| + | These parts contain ''crtZ'' (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in ''E. coli'' strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information]) | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 01:55, 27 October 2010
zeaxanthin synthesis operon (pBad+rbs+crtZ+plac+rbs +crtEBIY )
This part contains all five genes required, CrtEBIYZ, to produce zeaxanthin.
It is able to control the expression of crtZ by arabinose.
These parts contain crtZ (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 3810 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3346
Illegal NgoMIV site found at 3476
Illegal AgeI site found at 979
Illegal AgeI site found at 2561 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961