Difference between revisions of "Part:BBa K395702"
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It is necessary to add either beta-carotene or the synthesis operon. | It is necessary to add either beta-carotene or the synthesis operon. | ||
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| + | [[Image:tokyotech_Fig. 1.1.8.1 zeaxanthin pellet and plate.jpg|right|460px|Fig. 1.1.8.1 zeaxanthin pellet and plate]] | ||
| + | [[Image:tokyotech_table. 1.1.1.2.jpg|left|450px|figure1]] | ||
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| + | These parts contain ''crtZ'' (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in ''E. coli'' strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information]) | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 01:53, 27 October 2010
rbs+crtZ under pBad promoter for double plasmids
This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate.
It is necessary to add either beta-carotene or the synthesis operon.
These parts contain crtZ (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961