Difference between revisions of "Part:BBa B0031:Design"
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<partinfo>BBa_B0031 SequenceAndFeatures</partinfo> | <partinfo>BBa_B0031 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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+ | Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). | ||
− | + | No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. | |
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− | = | + | Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a> |
− | === | + | |
+ | ===Source=== | ||
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+ | ===References=== |
Latest revision as of 01:21, 5 June 2006
RBS.2 (weak) -- derivative of BBa_0030
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis).
No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>