Difference between revisions of "Part:BBa B0030:Design"
 
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<partinfo>BBa_B0030 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_B0030 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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Varies from -6 to +1 region from original sequence to accomodate BioBricks      suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis).   
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Varies from -6 to +1 region from original sequence to accomodate BioBricks      suffix ("orig" in figure 4-14 of Ron Weiss thesis).   
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No secondary structures are formed in the given RBS region. Users should      check for secondary structures induced in the RBS by upstream and downstream      elements in the +50 to -50 region, as such structures will greatly affect      the strength of the RBS.
 
No secondary structures are formed in the given RBS region. Users should      check for secondary structures induced in the RBS by upstream and downstream      elements in the +50 to -50 region, as such structures will greatly affect      the strength of the RBS.
  
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Contact info  <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
  
  
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===Source===
 
===Source===
  
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--What is the source of this part?--
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===References===
 
===References===
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===Files===
 

Latest revision as of 01:21, 5 June 2006


RBS.1 (strong) -- modified from R. Weiss


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis).

No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.

Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>


Source

References