Difference between revisions of "Part:BBa K305003:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The RBS sequence can be found in several ''B. subtilis'' genes and is known to efficiently initiate translation. The CDS was optimized for | + | The RBS sequence used for this part can be found in several ''B. subtilis'' genes and is known to efficiently initiate translation. The spacer sequence between the RBS and start codon originates form the ''B. subtilis'' wapA gene. The CDS was optimized for B. subtilis by the GenScript OptimumGeneTM software (see images). |
| + | [[Image:E-str-gn.jpg|200px|thumb|before optimization]][[Image:E-opt-gn.jpg|200px|thumb|after optimization]] | ||
===Structural Prediction=== | ===Structural Prediction=== | ||
Revision as of 00:31, 27 October 2010
Hydrophobic protein chaplin E (chpE)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The RBS sequence used for this part can be found in several B. subtilis genes and is known to efficiently initiate translation. The spacer sequence between the RBS and start codon originates form the B. subtilis wapA gene. The CDS was optimized for B. subtilis by the GenScript OptimumGeneTM software (see images).
Structural Prediction
Structural prediction of the protein without the signaling sequence by [http://zhanglab.ccmb.med.umich.edu/I-TASSER/ I-TASSER] yielded the following results. No tertiary structure has been identified by x-ray crystallography or NMR.
Source
Streptomyces coelicolor genomically encoded protein, codon optimized for B. subtilis and sythesized by Mr. Gene.
References
Claessen, D; Rink, R; de Jong, W et al. 2003. A novel class of secreted hydrophobic proteins is involved in aerial hyphae formation in Streptomyces coelicolor by forming amyloid-like fibrils. Genes Dev 17 1714-1726