Difference between revisions of "Part:BBa K349114:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | This part is a naked Red Fluorescent Protein. It does not include a promoter, an RBS, and start and stop codons. The promoter and terminator are intended to be reconstituted through adjoining linkers of the BioBytes 2.0 system. This part has built-in BsaI cutsites surrounding RFP which can produce A and B overhangs. | |
===Source=== | ===Source=== |
Revision as of 00:10, 27 October 2010
nRFP-AB (BioBytes 2.0)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 564
Illegal AgeI site found at 676 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 687
Design Notes
This part is a naked Red Fluorescent Protein. It does not include a promoter, an RBS, and start and stop codons. The promoter and terminator are intended to be reconstituted through adjoining linkers of the BioBytes 2.0 system. This part has built-in BsaI cutsites surrounding RFP which can produce A and B overhangs.
Source
pSB1C3