Difference between revisions of "Part:BBa K318501"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | RcsB is a transcription factor that is | + | RcsB is a transcription factor that is known to be a positive regulator of capsular polysaccharide synthesis. Along with transcription factor RcsA, the two transcription factors form a heterodimer that is know to activate around 19 genes related to colonic acid synthesis. RcsB is also know to form a homodimmer and positively regulate cell division. |
See more on [[http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10821 Ecocyc-RcsB]] | See more on [[http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10821 Ecocyc-RcsB]] | ||
Revision as of 23:12, 26 October 2010
lacI pL + RBS + RcsB + TT
Our goals is to have each cell produce enough colonic acid to protect itself from the harsh acidic conditions of the stomach. We placed RcsA, RcsB, and a combination of the two under a IPTG inducible promoter to test both quantity of colonic acid produced and cell survivability. You can see our data by downloading the associated .zip file for any of these three devices: BBa_K318500(RcsA), BBa_K318501(RcsB), BBa_K318502(RcsA+RcsB)
Usage and Biology
RcsB is a transcription factor that is known to be a positive regulator of capsular polysaccharide synthesis. Along with transcription factor RcsA, the two transcription factors form a heterodimer that is know to activate around 19 genes related to colonic acid synthesis. RcsB is also know to form a homodimmer and positively regulate cell division.
See more on http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10821 Ecocyc-RcsB
Characterization
Zip File Download
(Testing Data Here)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]