Difference between revisions of "Part:BBa K404090"

Revision as of 22:49, 26 October 2010

[AAV2]-Rep40ex

Rep40

Rep 40

BioBrick Nr.	BBa_K404090
RFC standard	RFC 10
Requirement	pSB1C3
Source	pAAV_MCS
Submitted by	[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]

Rep40 in some minutes
• 40 kDa
• ATPase and helicase activity
• Involved in genome encapsidation
The smallest Rep protein (Rep40) possesses helicase and ATPase activity as well, but does not have strict requirements for DNA duplexes containing a 3´single-stranded end. Rep40 helicase activity requires bivalent ions such as Mg2+ or Mn2+ and is most active using ATP as substrate. Lacking the zinc finger domain, present in Rep52, Rep40 requires dimerization for functional helicase activity (Collaco, Kalman-Maltese, Smith, Dignam, & Trempe, 2003). Rep40/52 proteins are required for translocation of the single-stranded, viral genomes into the preformed capsids proceeding with the 3´end of the DNA (King, Dubielzig, Grimm, & Kleinschmidt, 2001).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 53
Illegal XhoI site found at 920
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 <h3>Rep40</h3>
 {| style="margin: 0px 0px 300px 20px; color: black; float: right;" cellpadding="6" cellspacing="1" border="2"
-! colspan="2" style="background:#009900;"|[https://parts.igem.org/Part:BBa_K404090 rep 40]
+! colspan="2" style="background:#009900;"|[https://parts.igem.org/Part:BBa_K404090 Rep 40]
 |-
 ! colspan="2"|[[Image:Freiburg10_ReplicationBricks 1.png|200px]]
 |-
 |'''BioBrick Nr.'''
-|[https://parts.igem.org/Part:BBa_K40490 BBa_K404090]
+|[https://parts.igem.org/Part:BBa_K404090 BBa_K404090]
 |-
 |'''RFC standard'''