Difference between revisions of "Part:BBa K320003"

Latest revision as of 21:48, 26 October 2010

Origin for Asaia and resistance to Tetracycline

This BioBrick consists of two origins of replication, one for Asaia and one for E. coli, (BBa_K320000) and a resistance to Tetracycline (BBa_P1005) . It was designed to be inserted into (BBa_I51020).

Usage and Biology

This BioBrick can be used in E.Coli and also in Asaia to make the bacteria resistant to tetracycline.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1166
Illegal AgeI site found at 1326
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 4

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K320003 short</partinfo>
-This is a constitutive promoter for E. coli and Asaia. It contains a promoter and a ribosomal binding site and both elements are the E. coli consensus sequences.
+This BioBrick consists of two origins of replication, one for Asaia and one for ''E. coli'', (<partinfo>BBa_K320000</partinfo>) and a resistance to Tetracycline (<partinfo>BBa_P1005</partinfo>) . It was designed to be inserted into (<partinfo>BBa_I51020</partinfo>).
 ===Usage and Biology===
-This BioBrick can be used in E.Coli and espacially in Asaia to make the bacteia resistance to tetraclyline.
+This BioBrick can be used in ''E.Coli'' and also in Asaia to make the bacteria resistant to tetracycline.
@@ Line 13: / Line 15: @@
-<!-- Uncomment this to enable Functional Parameter display
+<!-- Uncomment this to enable Functional Parameter display -->
 ===Functional Parameters===
 <partinfo>BBa_K320003 parameters</partinfo>
 <!-- -->