Difference between revisions of "Part:BBa K404256"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of <a href=http://www.ncbi.nlm.nih.gov/protein/153106>Staphylococcal Protein A</a>. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering. | ||
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| + | <div style="float:right; width:480px; height:auto; "><img src="https://static.igem.org/mediawiki/parts/9/98/Freiburg10_ViralBrick_motif_Z34C.png" width="460" | ||
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Revision as of 21:06, 26 October 2010
pCMV_[AAV2]-VP123ex (ViralBrick-587KO-Z34C-Spacer)
The Z34C antibody binding motif including a knockout of the natural HSPG tropism & spacer, inserted into the 453 loop of pCMV_[AAV2]-VP123
Usage and Biology
The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of Staphylococcal Protein A. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2396
Illegal XhoI site found at 698
Illegal XhoI site found at 884 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 665
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3039
Illegal SapI site found at 1833