Difference between revisions of "Part:BBa K404225"
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===Usage and Biology===
 
===Usage and Biology===
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The Biotinylation Acceptor Peptide (BAP) is a 15 amino acid long peptide identified by Schatz J., 1993 in an library screening approach. This peptide with the sequence 5' - GLNDIFEAQKIEWHE - 3' contains a central lysine that is specifically biotinylated by the prokaryotic enzyme biotin holenzyme synthetase, encoded in the BirA gene of E. coli. Specific biotinylation of this peptide sequence can be performed in vivo by contransfecting a plasmid with the BirA gene as described for the AAV in Arnold et al.; 2006 or by an in vitro coupling approach using the purified Escherichia coli enzyme biotin ligase (BirA).
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<div style="float:right; width:480px; height:auto; "><img src="https://static.igem.org/mediawiki/parts/a/a9/Freiburg10_ViralBrick_motif_BAP.png" width="460"
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Revision as of 19:52, 26 October 2010

[AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587-BAP)
The Biotinylation Acceptor peptide motif, inserted into the 587 loop of [AAV2]-Rep-VP123_p5-TATAless

Freiburg10 Rep-VP123 P5-TATA-less 587-BAP.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3611
    Illegal XhoI site found at 1913
    Illegal XhoI site found at 2099
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4197
    Illegal BsaI site found at 4379
    Illegal BsaI site found at 4416
    Illegal SapI site found at 3048