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[[Image:Freiburg10_Rep-VP123_P5-TATA-less 587KO-Z34C-Spacer.png|thumb|center|480px]]<br>
 
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===Usage and Biology===
 
===Usage and Biology===
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The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of  <a href=http://www.ncbi.nlm.nih.gov/protein/153106>Staphylococcal Protein A</a>. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.
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<div style="float:right; width:480px; height:auto; "><img src="https://static.igem.org/mediawiki/parts/9/98/Freiburg10_ViralBrick_motif_Z34C.png" width="460"
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Revision as of 19:49, 26 October 2010

[AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-Z34C-Spacer)
The Z34C antibody binding motif including a knockout of the natural HSPG tropism & Spacer, inserted into the 587 loop of [AAV2]-Rep-VP123_p5-TATAless

Freiburg10 Rep-VP123 P5-TATA-less 587KO-Z34C-Spacer.png

Usage and Biology

The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of Staphylococcal Protein A. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3611
    Illegal XhoI site found at 1913
    Illegal XhoI site found at 2099
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4254
    Illegal BsaI site found at 4436
    Illegal BsaI site found at 4473
    Illegal SapI site found at 3048