Difference between revisions of "Part:BBa K404105"

Revision as of 19:02, 26 October 2010

p40 promoter

The P40 promoter is derived from the adeno-associated virus serotype 2 (AAV2) genome, where it is located at 40 map units. It regulates the transcription of the capsid proteins VP1, VP2 and VP3(Labow, Hermonat, & Berns, 1986; Cassinotti, Weitzand, & Tratschin, 1988). Several sequence regions have been identified to be important for maximal promoter activity: Two Sp1 sites, which are located 250 (Sp1-50) and 270 (GGT-70) base pairs upstream of the transcriptional start point and to which Sp1 or Sp1-like proteins bind (Pereira & Muzyczka 1997). Referring to the virus genome, p40 can also be induced through transactivation by the Rep proteins. The Sp1-50, together with the CArG-140 site of the P19 promoter, are the main elements involved in this process. The Rep proteins, which bind to the Rep binding element in the terminal repeat or the P5 promoter, can induce P19 or P40 by interaction with their bound Sp1 proteins thereby forming a DNA-loop (Pereira & Muzyczka 1997). In addition to that, the TATA box, located at 230, is also required for P40 activity. Furthermore the ATF-80 and the AP1-40 elements are also important for maximal promoter induction (Pereira & Muzyczka 1997).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K404105 short</partinfo>
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+The P40 promoter is derived from the adeno-associated virus serotype 2 (AAV2) genome, where it is located at 40 map units. It regulates the transcription of the capsid proteins VP1, VP2 and VP3(Labow, Hermonat, & Berns, 1986; Cassinotti, Weitzand, & Tratschin, 1988).
+Several sequence regions have been identified to be important for maximal promoter activity: Two Sp1 sites, which are located 250 (Sp1-50) and 270 (GGT-70) base pairs upstream of the transcriptional start point and to which Sp1 or Sp1-like proteins bind (Pereira & Muzyczka 1997).
+Referring to the virus genome, p40 can also be induced through transactivation by the Rep proteins. The Sp1-50, together with the CArG-140 site of the P19 promoter, are the main elements involved in this process. The Rep proteins, which bind to the Rep binding element in the terminal repeat or the P5 promoter, can induce P19 or P40 by interaction with their bound Sp1 proteins thereby forming a DNA-loop (Pereira & Muzyczka 1997). In addition to that, the TATA box, located at 230, is also required for P40 activity. Furthermore the ATF-80 and the AP1-40 elements are also important for maximal promoter induction (Pereira & Muzyczka 1997).
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