Difference between revisions of "Part:BBa K411101"

 
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Fused splits of the first half of [[Part:BBa_E0040|GFP]] and the first half of [[Part:BBa_K411100|eIF4A]] with a GSSGSSGSGS peptide linker in the middle.
 
Fused splits of the first half of [[Part:BBa_E0040|GFP]] and the first half of [[Part:BBa_K411100|eIF4A]] with a GSSGSSGSGS peptide linker in the middle.
 +
For more details of the design, please see
 +
NYMU-Taipei's [http://2010.igem.org/Team:NYMU-Taipei/Project/Speedy_reporter/Materials_and_Methods#GFP_fusion_part wiki page].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:14, 26 October 2010

GFP_split_A + Linker + eIF4A_split_A

Fused splits of the first half of GFP and the first half of eIF4A with a GSSGSSGSGS peptide linker in the middle. For more details of the design, please see NYMU-Taipei's [http://2010.igem.org/Team:NYMU-Taipei/Project/Speedy_reporter/Materials_and_Methods#GFP_fusion_part wiki page].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 741
    Illegal BglII site found at 1037
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1139