Difference between revisions of "Part:BBa K374008:Experience"
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The biobrick was cloned into the pSB4A5 plasmid, and a GFP reporter gene was inserted downstream of it. The plasmid was transformed into ''E.coli'' (DH5 alpha) and measurements were done on this strain using a BioLector microreactor system. The BioLector is a microfermenting system that measures OD and fluorescence simultaneously. | The biobrick was cloned into the pSB4A5 plasmid, and a GFP reporter gene was inserted downstream of it. The plasmid was transformed into ''E.coli'' (DH5 alpha) and measurements were done on this strain using a BioLector microreactor system. The BioLector is a microfermenting system that measures OD and fluorescence simultaneously. | ||
Revision as of 16:48, 26 October 2010
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Applications of BBa_K374008
Biolector experiment<h3>
The biobrick was cloned into the pSB4A5 plasmid, and a GFP reporter gene was inserted downstream of it. The plasmid was transformed into E.coli (DH5 alpha) and measurements were done on this strain using a BioLector microreactor system. The BioLector is a microfermenting system that measures OD and fluorescence simultaneously.
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