Difference between revisions of "Part:BBa K404213"
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===Restriction sites=== | ===Restriction sites=== | ||
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Revision as of 16:19, 26 October 2010
ViralBrick-587KO-Z34C
The Z34C antibody binding motif including a knockout of the natural HSPG tropism, ready for insertion into the 587 loop
Usage and Biology
The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of [http://www.ncbi.nlm.nih.gov/protein/153106 Staphylococcal Protein A]. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.
Restriction sites
| Restriction site | Enzyme | Recognition sequence | Activity | Temp. |
|---|---|---|---|---|
| upstream 587 | [http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF] |  |
100;50;10;100 | 37°C |
| downstream 587 | [http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF] |  |
0;25;0;100 | 37°C |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 10
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]