Difference between revisions of "Part:BBa K404205"
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[[Image:Freiburg10_ViralBrick-logo-587-BAP.png|thumb|center|480px]]<br>
 
[[Image:Freiburg10_ViralBrick-logo-587-BAP.png|thumb|center|480px]]<br>
  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
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 +
===Restriction sites===
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<table border="1" style="float:right; width:480px; height:auto;>
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<tr>
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  <th><b>Restriction site</b></th>
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  <th><b>Enzyme</b></th>
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  <th><b>Recognition sequence</b></th>
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  <th><b>Activity</b></th>
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  <th><b>Temp.</b></th>
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</tr>
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<tr>
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  <td>upstream 453</td>
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  <td>[http://www.neb.com/nebecomm/products/productR3132.asp SspI-HF]</td>
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  <td>[[image:Freiburg10_recognition site SspI-HF.Gif]]</td>
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  <td>25;100;0;100</td>
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<td>37°C</td>
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</tr>
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<tr>
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  <td>downstream 453</td>
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  <td>[http://www.neb.com/nebecomm/products/productR3138.asp SalI-HF]</td>
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  <td>[[image:Freiburg10_recognition site SalI-HF.Gif]]</td>
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  <td>10;100;100;100</td>
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  <td>37°C</td>
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<tr>
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</table>
  
 
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Revision as of 15:48, 26 October 2010

ViralBrick-587-BAP
The Biotinylation Acceptor peptide motif, ready for insertion into the 587 loop

Freiburg10 ViralBrick-logo-587-BAP.png

Usage and Biology

Restriction sites

Restriction site Enzyme Recognition sequence Activity Temp.
upstream 453 [http://www.neb.com/nebecomm/products/productR3132.asp SspI-HF] Freiburg10 recognition site SspI-HF.Gif 25;100;0;100 37°C
downstream 453 [http://www.neb.com/nebecomm/products/productR3138.asp SalI-HF] Freiburg10 recognition site SalI-HF.Gif 10;100;100;100 37°C

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]