Difference between revisions of "Part:BBa K323138:Design"
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<partinfo>BBa_K323138 short</partinfo> | <partinfo>BBa_K323138 short</partinfo> | ||
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Carotenoid biosynthesis operon. Comprises of beta-carotene biosynthesis operon from P. ananatis and synthetic crtO (beta carotene ketolase) gene with Elowitz RBS. The operon is placed under lacI promoter (Bba_r0011) | Carotenoid biosynthesis operon. Comprises of beta-carotene biosynthesis operon from P. ananatis and synthetic crtO (beta carotene ketolase) gene with Elowitz RBS. The operon is placed under lacI promoter (Bba_r0011) | ||
+ | Presumably synthesises echinenon (not confirmed). For canthaxanthin biosynthesis crtO overexpression is required. | ||
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<partinfo>BBa_K323138 SequenceAndFeatures</partinfo> | <partinfo>BBa_K323138 SequenceAndFeatures</partinfo> | ||
Latest revision as of 15:25, 26 October 2010
R0011 crtEBIYO
Carotenoid biosynthesis operon. Comprises of beta-carotene biosynthesis operon from P. ananatis and synthetic crtO (beta carotene ketolase) gene with Elowitz RBS. The operon is placed under lacI promoter (Bba_r0011) Presumably synthesises echinenon (not confirmed). For canthaxanthin biosynthesis crtO overexpression is required.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 5708
Illegal BamHI site found at 2037
Illegal BamHI site found at 5834 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1573
Illegal NgoMIV site found at 1703
Illegal AgeI site found at 788 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
a
Source
a