Difference between revisions of "Part:BBa K362052:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K362052 short</partinfo>
 
<partinfo>BBa_K362052 short</partinfo>
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===Design Notes===
 
===Design Notes===
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This composite part contains ahpC promoter(BBa_K362001),RBS(BBa_K121013),GFP(BBa_K121013),2 terminators(BBa_K121013),sufA promoter(BBa_K362005),RBS(BBa_I13507),RFP(BBa_I13507) and 2 terminators(BBa_I13507). sufA promoter has sensitibity to oxydative stress.
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This composite part contains ahpC promoter(BBa_K362001),RBS(BBa_I13507),RFP(BBa_I13507) and 2 terminators(BBa_I13507),RBS(BBa_K121013),GFP(BBa_K121013),2 terminators(BBa_K121013),sufA promoter(BBa_K362005),RBS(BBa_K121013),GFP(BBa_K121013),2 terminators(BBa_K121013). sufA promoter has sensitibity to oxydative stress.
 
ahpC promoter is controlled by OxyR protein, an oxidative stress resoponsive transcription facor.  
 
ahpC promoter is controlled by OxyR protein, an oxidative stress resoponsive transcription facor.  
 
sufA promoter has sensitivity to oxidative stress.
 
sufA promoter has sensitivity to oxidative stress.
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===Source===
 
===Source===

Latest revision as of 15:16, 26 October 2010

ahpC promoter - RBS - RFP - Term - Term - sufA promoter - RBS - GFP - Term - Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 780
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 780
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2911
    Illegal BamHI site found at 2900
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 780
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 780
    Illegal AgeI site found at 1581
    Illegal AgeI site found at 1693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3603


Design Notes

This composite part contains ahpC promoter(BBa_K362001),RBS(BBa_I13507),RFP(BBa_I13507) and 2 terminators(BBa_I13507),RBS(BBa_K121013),GFP(BBa_K121013),2 terminators(BBa_K121013),sufA promoter(BBa_K362005),RBS(BBa_K121013),GFP(BBa_K121013),2 terminators(BBa_K121013). sufA promoter has sensitibity to oxydative stress. ahpC promoter is controlled by OxyR protein, an oxidative stress resoponsive transcription facor. sufA promoter has sensitivity to oxidative stress.

Source

sufA Promoter from E.coli genome

References