Difference between revisions of "Part:BBa K404229"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K404229 short</partinfo>
 
<partinfo>BBa_K404229 short</partinfo>
−
<br><b>The Biotinylation Acceptor peptide motif, ready for insertion into the 453 loop</b>
+
<br><b>The Biotinylation Acceptor peptide motif, including a knockout of the natural HSPG tropism, inserted into the 587 loop of [[Part:BBa_K404002|[AAV2]-Rep-VP123_p5-TATAless]]</b>
  
 
[[Image:Freiburg10_Rep-VP123_P5-TATA-less 587KO-BAP.png|thumb|center|480px]]<br>
 
[[Image:Freiburg10_Rep-VP123_P5-TATA-less 587KO-BAP.png|thumb|center|480px]]<br>

Revision as of 12:26, 26 October 2010

[AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-BAP)
The Biotinylation Acceptor peptide motif, including a knockout of the natural HSPG tropism, inserted into the 587 loop of [AAV2]-Rep-VP123_p5-TATAless

Freiburg10 Rep-VP123 P5-TATA-less 587KO-BAP.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3611
    Illegal XhoI site found at 1913
    Illegal XhoI site found at 2099
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4197
    Illegal BsaI site found at 4379
    Illegal BsaI site found at 4416
    Illegal SapI site found at 3048