Difference between revisions of "Part:BBa K422014"

Revision as of 10:29, 26 October 2010

mCyPet (AarI A-part)

Biological Background

CyPet is an evolutionary optimized cyan fluorescent protein CFP developed in 2005 by Nguyen and Daugherty. They showed the FRET pair CyPet-YPet to have a 7-fold higher ratiometric FRET signal change than their parental pair.

mCyPet has a mutation in A206K preventing it from forming dimers (Ohashi et al., 20007).

Cloning strategy

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. cyPet-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 733
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 733
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 733
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 733
Illegal NgoMIV site found at 697
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 ===Biological Background===
-CFP variant.
+CyPet is an evolutionary optimized cyan fluorescent protein CFP developed in 2005 by Nguyen and Daugherty. They showed the FRET pair CyPet-YPet to have a 7-fold higher ratiometric FRET signal change than their parental pair.
+mCyPet has a mutation in A206K preventing it from forming dimers (Ohashi ''et al.,'' 20007).
 ===Cloning strategy===