Difference between revisions of "Part:BBa K337019:Design"

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===References===
 
===References===
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http://tools.invitrogen.com/content/sfs/manuals/pcdna5frtto_man.pdf

Latest revision as of 08:56, 26 October 2010

FRT site with spacing


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 155
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 155
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 155
    Illegal NgoMIV site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

as the FRT site is a pretty short sequence we added some spacing in front and behind to enable easy cloning of this part.


Source

pcDNA5/FRT/TO (Invitrogen)

References

http://tools.invitrogen.com/content/sfs/manuals/pcdna5frtto_man.pdf