Difference between revisions of "Part:BBa K337019:Design"
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===Source=== | ===Source=== | ||
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===References=== | ===References=== |
Revision as of 08:56, 26 October 2010
FRT site with spacing
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 155
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 155
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 155
Illegal NgoMIV site found at 15 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
as the FRT site is a pretty short sequence we added some spacing in front and behind to enable easy cloning of this part.
Source
pcDNA5/FRT/TO (Invitrogen)