Difference between revisions of "Part:BBa K238008"

Latest revision as of 06:41, 26 October 2010

VirA

This is VirA, a histidine kinase sensor originally found in Agrobacterium tumefaciens, Ti plasmid. It is part of the two-component VirA/VirG signal transduction system. This system is able to sense phenolic compounds and regulate gene transcription correspondingly.

VirA has illegal PstI restriction sites in its sequence. We used site directed mutagenesis to get these sites out. PstI was mutated from CTGCAG to CTGCAA.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 502
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 666
Illegal BglII site found at 1559
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1405
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1177
Illegal BsaI site found at 1969
Illegal SapI site found at 1651
Illegal SapI.rc site found at 880

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K238008 short</partinfo>
-This is Vir A, a sensor originally found in Agrobacterium tumefaciens, plasmid Ti. It is able to sense Phenols and as a reaction regulate gene transcription.
+This is VirA, a histidine kinase sensor originally found in ''Agrobacterium tumefaciens'', Ti plasmid. It is part of the two-component VirA/VirG signal transduction system. This system is able to sense phenolic compounds and regulate gene transcription correspondingly.
+VirA has illegal PstI restriction sites in its sequence. We used site directed mutagenesis to get these sites out. PstI was mutated from CTGCAG to CTGCAA.
 <!-- Add more about the biology of this part here