Difference between revisions of "Part:BBa K302012:Design"

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===Source===
 
===Source===
  
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Genomic DNA from ''Bacillus subtilis'' -''yneA''
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* ''yneA'': Genomic DNA from ''Bacillus subtilis''
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Hyperspankoid from oid and hyperspank  
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* Hyperspankoid from pspac, oid and hyperspank
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===References===
 
===References===
  
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Kawai, Y., Moriya, S., & Ogasawara, N. (2003). Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis. Molecular microbiology, 47(4), 1113-22. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12581363.
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Kawai, Y., Moriya, S., & Ogasawara, N. (2003). Identification of a protein, YneA, responsible for cell division suppression during the SOS response in ''Bacillus subtilis''. Molecular microbiology, 47(4), 1113-22. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12581363.
  
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Mo, A.H. & Burkholder, W.F., 2010. YneA , an SOS-Induced Inhibitor of Cell Division in Bacillus subtilis , Is Regulated Posttranslationally and Requires the Transmembrane Region for Activity ᰔ †. Society, 192(12), 3159-3173.
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Mo, A.H. & Burkholder, W.F., 2010. YneA , an SOS-Induced Inhibitor of Cell Division in ''Bacillus subtilis'' , Is Regulated Posttranslationally and Requires the Transmembrane Region for Activity ᰔ †. Society, 192(12), 3159-3173.

Latest revision as of 02:08, 26 October 2010

IPTG-inducible filamentous cell formation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The stop codon was modified to a double TAA.

Source

  • yneA: Genomic DNA from Bacillus subtilis
  • Hyperspankoid from pspac, oid and hyperspank

References

Kawai, Y., Moriya, S., & Ogasawara, N. (2003). Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis. Molecular microbiology, 47(4), 1113-22. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12581363.

Mo, A.H. & Burkholder, W.F., 2010. YneA , an SOS-Induced Inhibitor of Cell Division in Bacillus subtilis , Is Regulated Posttranslationally and Requires the Transmembrane Region for Activity ᰔ †. Society, 192(12), 3159-3173.