Difference between revisions of "Part:BBa K364303:Experience"

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how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
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===Physiological effect===
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===Ligand===
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daf-12 in Caenorhabditis elegans plays a key role in the regulation of development and determination of adult longevity. It has effects also on resistance to oxidative and thermal stress which makes daf-12 the first gene with alleles that can extend or shorten lifespan.  
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The ligands of DAF-12 are various isomers of dafachronic acids, which derive from sterols upon oxidation by DAF-9, a cytochrome P450 enzime.
  
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===Physiological effects of DAF-12===
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daf-12 in Caenorhabditis elegans plays a key role in the regulation of development and determination of adult longevity. It has effects also on resistance to oxidative and thermal stress which makes daf-12 the first gene with alleles that can extend or shorten lifespan.
  
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===Applications of BBa_K364303===
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===Possible applications od DAF-12 LBD===
 
DAF-12 LBD can be fused with various DBDs to activate the expression of chosen genes by addition of dafachronic acids (ligand).
 
DAF-12 LBD can be fused with various DBDs to activate the expression of chosen genes by addition of dafachronic acids (ligand).
  
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===References===
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===Applications of BBa_K364303===
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Antebi A, Yeh WH, Tait D, Hedgecock EM, Riddle DL. daf-12 encodes a nuclear receptor that regulates the dauer diapause and developmental age in C. elegans. Genes Dev. 2000 Jun 15;14(12):1512-27.
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Snow MI, Larsen PL.Structure and expression of daf-12: a nuclear hormone receptor with three isoforms that are involved in development and aging in Caenorhabditis elegans. Biochim Biophys Acta. 2000 Nov 15;1494(1-2):104-16.
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Matyash V, Entchev EV, Mende F, Wilsch-Bräuninger M, Thiele C, et al. (2004) Sterol-Derived Hormone(s) Controls Entry into Diapause in Caenorhabditis elegans by Consecutive Activation of DAF-12 and DAF-16. PLoS Biol 2(10): e280.
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Motola D.L., Cummins C.L., Rottiers V., Sharma K.K., Li T., Li Y., Suino-Powell K., (...), Mangelsdorf D.J. Identification of Ligands for DAF-12 that Govern Dauer Formation and Reproduction in C. elegans. (2006) Cell, 124 (6), pp. 1209-1223.
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===User Reviews===
 
===User Reviews===

Latest revision as of 01:45, 26 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Ligand

The ligands of DAF-12 are various isomers of dafachronic acids, which derive from sterols upon oxidation by DAF-9, a cytochrome P450 enzime.

Physiological effects of DAF-12

daf-12 in Caenorhabditis elegans plays a key role in the regulation of development and determination of adult longevity. It has effects also on resistance to oxidative and thermal stress which makes daf-12 the first gene with alleles that can extend or shorten lifespan.

Possible applications od DAF-12 LBD

DAF-12 LBD can be fused with various DBDs to activate the expression of chosen genes by addition of dafachronic acids (ligand).

Applications of BBa_K364303

User Reviews

UNIQbdaba07d4ef1316f-partinfo-00000000-QINU UNIQbdaba07d4ef1316f-partinfo-00000001-QINU