Difference between revisions of "Part:BBa K364328:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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| + | ===Ligand=== | ||
| + | The ligands of DAF-12 are various isomers of dafachronic acids, which derive from sterols upon oxidation by DAF-9, a cytochrome P450 enzime. | ||
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| + | ===Physiological effects of DAF-12=== | ||
| + | daf-12 in Caenorhabditis elegans plays a key role in the regulation of development and determination of adult longevity. It has effects also on resistance to oxidative and thermal stress which makes daf-12 the first gene with alleles that can extend or shorten lifespan. | ||
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| + | ===Possible applications of GAL4-DAF-12=== | ||
| + | By the addition of lipid ligands (dafachronic acids), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase). | ||
===Applications of BBa_K364328=== | ===Applications of BBa_K364328=== | ||
| − | [[Image:Team Debrecen pCDNA-Gal4-DAF12.jpg|800px|thumb|center|Picture of gel electrophoresis: Gal4-DAF12 in pCDNA3.1 resulting an insert of | + | [[Image:Team Debrecen pCDNA-Gal4-DAF12.jpg|800px|thumb|center|Picture of gel electrophoresis: Gal4-DAF12 in pCDNA3.1 resulting an insert of 971 bp.]] |
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 01:44, 26 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Ligand
The ligands of DAF-12 are various isomers of dafachronic acids, which derive from sterols upon oxidation by DAF-9, a cytochrome P450 enzime.
Physiological effects of DAF-12
daf-12 in Caenorhabditis elegans plays a key role in the regulation of development and determination of adult longevity. It has effects also on resistance to oxidative and thermal stress which makes daf-12 the first gene with alleles that can extend or shorten lifespan.
Possible applications of GAL4-DAF-12
By the addition of lipid ligands (dafachronic acids), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).
Applications of BBa_K364328
User Reviews
UNIQ45f692cf7622a82d-partinfo-00000000-QINU UNIQ45f692cf7622a82d-partinfo-00000001-QINU