Difference between revisions of "Part:BBa K302035:Design"
PhilipHall (Talk | contribs) (New page: __NOTOC__ <partinfo>BBa_K302035 short</partinfo> <partinfo>BBa_K302035 SequenceAndFeatures</partinfo> ===Design Notes=== Sequence selected based on the work of Pellegrini at al. and Shi...) |
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===Design Notes=== | ===Design Notes=== | ||
| − | Sequence selected based on the work of Pellegrini at al. and Shimotsu and Henner in the papers below. | + | *Sequence selected based on the work of Pellegrini at al. and Shimotsu and Henner in the papers below. |
| + | *Organisms with this device integrated must be grown on 10% sucrose media to be viable. | ||
| + | *Should be integrated at the ''sacB'' locus to prevent native Levansucrose glue production. | ||
===Source=== | ===Source=== | ||
Revision as of 01:14, 26 October 2010
Sucrose-limitation induced kill switch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 363
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- Sequence selected based on the work of Pellegrini at al. and Shimotsu and Henner in the papers below.
- Organisms with this device integrated must be grown on 10% sucrose media to be viable.
- Should be integrated at the sacB locus to prevent native Levansucrose glue production.
Source
Bacillus Subtilis 168 strain
References
Pellegrini, O., Mathy, N., Gogos, A., Shapiro, L., and Condon, C. (2005) The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. Mol Microbiol 56: 1139–1148.
Shimotsu, H., and Henner, D. J. (1986) J. Bacteriol. 168, 380–388