Difference between revisions of "Template:ExpressIt"

Latest revision as of 23:41, 25 October 2010

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Creative BioCommons

This is expression vector containing {{{RBS}}} under {{{promoter}}} promoter. It can be used for easily expression of any protein-encoding BioBrick in RFC10 format.

Authors

Clonning:

Ania Puławska
Milena Bażlekowa

The lab work was supervised by:

Michał Lower
Ania Olchowik

Construct design

The device consists of {{{promoter}}} promoter followed by {{{RBS}}} RBS. Device was created by annealing of synthetic complementary oligonucleotides followed by subsequent cloning into pSB1A2 vector.

Expression strength

Expression strength of this device is based on measurements of [http://2010.igem.org/Team:Warsaw/Stage1/PromMeas promoter strength] and [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS performance] done by Team Warsaw 2010.

It's estimated to be {{{strength}}} relative to BBa_K299509, the strongest ExpressIt! vector.

Summary for the whole ExpressIt! family is shown below:

@@ Line 19: / Line 19: @@
 Expression strength of this device is based on measurements of [http://2010.igem.org/Team:Warsaw/Stage1/PromMeas promoter strength] and [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS performance] done by Team Warsaw 2010.
-It's estimated to be '''{{{strength}}}''' relative to <partinfo>BBa_K299504</partinfo>, the strongest ExpressIt! vector.
+It's estimated to be '''{{{strength}}}''' relative to <partinfo>BBa_K299509</partinfo>, the strongest ExpressIt! vector.
 Summary for the whole ExpressIt! family is shown below:
 https://static.igem.org/mediawiki/2010/0/09/ExpressIt.png