Difference between revisions of "Part:BBa K299020"
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Latest revision as of 22:46, 25 October 2010
RBS measurement device pT7 B0030 GFP
This part is licensed under
Creative BioCommons
We planned to use this device to determine BBa_B0030 strength relative to BBa_B0034 using cell-free system.
Authors
Clonning:
- Ania Puławska
- Milena Bażlekowa
The lab work was supervised by:
- Michał Lower
- Ania Olchowik
Construct design
The device consists of BBa_I719005 T7 promoter followed by BBa_B0030 community RBS and BBa_I13401 reporter module. GFPmut3b was used as reporter protein. Device was created by cloning BBa_I13401 behind ExpressIt! vector containing appropriate RBS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 696