Difference between revisions of "Part:BBa K364321:Design"

 
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<partinfo>BBa_K364321 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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The reconstruction of the DBD to the hinge region, LBD and Backbone will be done with RFC 25.
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The reconstruction of the LBD and Backbone will be done with RFC 25.
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===Source===
 
===Source===
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Arteficially synthetized Ligand Binding domain of C. elegans nuclear receptor NHR-64. This part is placed into pSB1C3 carrier vector.
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C. elegans orphan nuclear receptor
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===References===
 
===References===
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Liang B,  Ferguson K,  Kadyk L,  Watts JL, 2010. The Role of Nuclear Receptor NHR-64 in Fat Storage Regulation in Caenorhabditis elegans. PLoS ONE 5(3): e9869. doi:10.1371/journal.pone.0009869

Latest revision as of 22:01, 25 October 2010

NHR-64 LBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 178


Design Notes

The reconstruction of the LBD and Backbone will be done with RFC 25.

Source

Arteficially synthetized Ligand Binding domain of C. elegans nuclear receptor NHR-64. This part is placed into pSB1C3 carrier vector.

References

Liang B, Ferguson K, Kadyk L, Watts JL, 2010. The Role of Nuclear Receptor NHR-64 in Fat Storage Regulation in Caenorhabditis elegans. PLoS ONE 5(3): e9869. doi:10.1371/journal.pone.0009869