Difference between revisions of "Part:BBa K302012:Experience"

(Applications of BBa_K302012)
(Applications of BBa_K302012)
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|'''Graph4''':
 
|'''Graph4''':
 
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|[[Image:newcastle_1IPTG.jpg|500px]]
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|'''See below''': ''Bacillus subtilis 168'' cells (left) and cells induced at 1mM IPTG(right)
 
|'''See below''': ''Bacillus subtilis 168'' cells (left) and cells induced at 1mM IPTG(right)

Revision as of 21:44, 25 October 2010

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Applications of BBa_K302012

Graph1:
Teamnewcastle yneA168.png
Graph 1 shows that overexpression of the yneA gene (ΔamyE:pSpac(hy)-oid::yneA) leads to a longer cell length compared with our control Bacillus subtilis 168.
Graph2:
Newcastle no induction.jpg
See below: Bacillus subtilis 168 cells (left) and non-induced cells(right)
Teamnewcastle yneA168BS.jpgTeamnewcastle noindBS.jpg
Graph 2 shows the percentage of cells at different lengths(μm)uninduced
Graph3:
Newcastle 0.2 induction.jpg
See below: Bacillus subtilis 168 cells (left) and cells induced at 0.2mM IPTG(right)
Teamnewcastle yneA168BS.jpgTeamnewcastle 0.2indBS.jpg
Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG
Graph4:
Newcastle 1IPTG.jpg
See below: Bacillus subtilis 168 cells (left) and cells induced at 1mM IPTG(right)
Teamnewcastle yneA168BS.jpgTeamnewcastle 1indBS2.jpg
Graph 4 shows the percentage of cells at different lengths(μm)induced at 1mM IPTG

Graphs 2,3 and 4 show a greater proportion of cells at a higher concentration of IPTG(1mM IPTG), compared with Bacillus subtilis 168 our control population.

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