Difference between revisions of "Part:BBa K364317:Design"

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===Design Notes===
 
===Design Notes===
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The reconstruction of the DBD to the hinge region, LBD and Backbone will be done with RFC 25
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The construction of the LBD and pSB1C3 backbone have been done with RFC 25.
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+
−
 
+
  
 
===Source===
 
===Source===
  
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C. elegans orphan nuclear receptor  
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Arteficially synthetized Ligand Binding Domain of C. elegans NHR-31 nuclear receptor, placed into pSB1C3 carrier vector.
  
 
===References===
 
===References===
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1. Hahn-Windgassen A, Van Gilst MR."The Caenorhabditis elegans HNF4alpha Homolog, NHR-31, mediates excretory tube growth and function through coordinate regulation of the vacuolar ATPase."[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720251/?tool=pubmed] PLoS Genet. 2009 Jul;5(7):e1000553. Epub 2009 Jul 10.
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1. Hahn-Windgassen A, Van Gilst MR."The Caenorhabditis elegans HNF4alpha Homolog, NHR-31, mediates excretory tube growth and function through coordinate regulation of the vacuolar ATPase." [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720251/?tool=pubmed PLoS Genet.2009 Jul;5(7):e1000553. Epub 2009 Jul 10.]

Latest revision as of 20:22, 25 October 2010

NHR-31 LBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 229
    Illegal SapI.rc site found at 99


Design Notes

The construction of the LBD and pSB1C3 backbone have been done with RFC 25.

Source

Arteficially synthetized Ligand Binding Domain of C. elegans NHR-31 nuclear receptor, placed into pSB1C3 carrier vector.

References

1. Hahn-Windgassen A, Van Gilst MR."The Caenorhabditis elegans HNF4alpha Homolog, NHR-31, mediates excretory tube growth and function through coordinate regulation of the vacuolar ATPase." [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720251/?tool=pubmed PLoS Genet.2009 Jul;5(7):e1000553. Epub 2009 Jul 10.]