Difference between revisions of "Part:BBa K415300"
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| − | From <i>Programmable cells: Interfacing natural and engineered gene networks</i>, PNAS. [[Image:CollinsToggle.png|frame|150px|right|The original pTSMa plasmid from the Collins paper. The parts have since been biobricked into pSB1C3.]] | + | From <i>Programmable cells: Interfacing natural and engineered gene networks</i>, Kobayashi, H. et. al, PNAS. [[Image:CollinsToggle.png|frame|150px|right|The original pTSMa plasmid from the Collins paper. The parts have since been biobricked into pSB1C3.]] |
The [http://www.pnas.org/content/101/22/8414.full|Collins/Kobayashi UV Toggle] (2004) biobricked. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.<br><br> | The [http://www.pnas.org/content/101/22/8414.full|Collins/Kobayashi UV Toggle] (2004) biobricked. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.<br><br> | ||
Revision as of 20:05, 25 October 2010
Collins UV Toggle - Ptrc:cI:tt:Plambda:lacI:tt
From Programmable cells: Interfacing natural and engineered gene networks, Kobayashi, H. et. al, PNAS.
The original pTSMa plasmid from the Collins paper. The parts have since been biobricked into pSB1C3.
The [http://www.pnas.org/content/101/22/8414.full|Collins/Kobayashi UV Toggle] (2004) biobricked. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.
The following data was taken from cells co-transformed with pTSMa and Part:BBa_K415023.
| UV Power Modulation | ||||
|---|---|---|---|---|
| Photos | 
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| FACS | Image | |||
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1773
- 1000COMPATIBLE WITH RFC[1000]