Difference between revisions of "Part:BBa K323088:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | a | + | Primers for the synthetic promoter pSYN containing a ''Bbs''I restriction site were designed. After annealing, the resulting fragment was ligated into a vector containing a double terminator ([[Part:BBa_B0015]]) as a back insert. |
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===Source=== | ===Source=== | ||
| − | + | The double terminator ([[Part:BBa_B0015]]) was taken from the Registry of parts and the primers for the synthetic promoter were ordered from Sigma-Aldrich. | |
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===References=== | ===References=== | ||
Latest revision as of 19:31, 25 October 2010
In vivo testing device for protein-DNA binding: part 1 (DTER_pSYN_BbsI)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers for the synthetic promoter pSYN containing a BbsI restriction site were designed. After annealing, the resulting fragment was ligated into a vector containing a double terminator (Part:BBa_B0015) as a back insert.
Source
The double terminator (Part:BBa_B0015) was taken from the Registry of parts and the primers for the synthetic promoter were ordered from Sigma-Aldrich.