Difference between revisions of "Part:BBa K320003"
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<partinfo>BBa_K320003 short</partinfo>
 
<partinfo>BBa_K320003 short</partinfo>
  
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This is a constitutive promoter for E. coli and Asaia. It contains a promoter and a ribosomal binding site and both elements are the E. coli consensus sequences.
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This BioBrick is constitued by an origin of replication for Asaia and E. coli (BBa_K32000) and a resistance to Tetracycline <partinfo>BBa_P1005</partinfo> BBa_P1005). It was initially designed to be inserted into BBa_I51020. (make links out of all biobrick references)
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===Usage and Biology===
 
===Usage and Biology===
 
This BioBrick can be used in E.Coli and espacially in Asaia to make the bacteia resistance to tetraclyline.  
 
This BioBrick can be used in E.Coli and espacially in Asaia to make the bacteia resistance to tetraclyline.  

Revision as of 19:17, 25 October 2010

Origin for Asaia and resistance to Tetracycline

This BioBrick is constitued by an origin of replication for Asaia and E. coli (BBa_K32000) and a resistance to Tetracycline BBa_P1005 BBa_P1005). It was initially designed to be inserted into BBa_I51020. (make links out of all biobrick references)

Usage and Biology

This BioBrick can be used in E.Coli and espacially in Asaia to make the bacteia resistance to tetraclyline.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1166
    Illegal AgeI site found at 1326
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4